Immortalized Porcine Skin Fibroblasts-SV40

In the realm of biomedical research, the demand for reliable and robust cell lines has never been greater. Among various cell types, immortalized porcine skin fibroblasts, particularly those transformed by simian virus 40 (SV40), have gained significant attention. These cells offer numerous advantages in studying cellular behaviors, drug responses, and various biological processes relevant to veterinary and human medicine. What are Immortalized Porcine Skin Fibroblasts?Fibroblasts are a type of cell found in connective tissue that plays a crucial role in the maintenance of the extracellular matrix and tissue repair. Porcine (pig) skin fibroblasts are especially valuable due to the physiological similarities they share with human cells. However, primary fibroblast cultures have a limited lifespan due to replicative senescence. To overcome this limitation, scientists have developed immortalized porcine skin fibroblasts using SV40. SV40 is a simian virus that has been widely used in cell biology for its ability to induce cellular transformation and bypass normal cell cycle controls. By introducing the SV40 large T antigen into porcine skin fibroblasts, researchers can create cell lines that can proliferate indefinitely while retaining many properties of the original cells.